Update on Commonly Used Molecular Typing Methods for Clostridioides difficile

This review aims to provide a comprehensive overview of the significant Clostridioides difficile molecular typing techniques currently employed in research and medical communities. The main objectives this are describe key methods utilized C. studies highlight epidemiological characteristics most prevalent strains on global scale. Geographically distinct regions exhibit strain types difficile, with notable concordance observed among various methodologies. advantages that next-generation sequencing (NGS) offers has changed epidemiology research, enabling high-resolution genomic analyses pathogen. NGS platforms offer an unprecedented opportunity explore genetic intricacies evolutionary trajectories strains. It is relevant acknowledge novel routes transmission continually being unveiled warrant further investigation, particularly context zoonotic implications environmental contamination.